维生素C调控肉鸡缺氧肺动脉血管平滑肌细胞缺氧诱导因子-1α mRNA转录的分子机制

doi:10.11843/j.issn.0366-6964.2016.05.022

Abstract

Abstract:

This study was conducted to investigate the molecular mechanism of Vitamin C (VC) on redox state and the mRNA transcription of hypoxia inducible factor-1α(HIF-1α)，Vascular endothelial growth factor (VEGF) and Vascular endothelial growth factor receptor 2 (VEGFR2/Flk-1) in broiler PASMCs under hypoxia condition through dimethyloxalylglycine (DMOG) and H₂O₂.This study executed on the basis of the culture of PASMCs and hypoxia model，included 3 experiments，VC and H₂O₂，VC and DMOG，VC and H₂O₂+DMOG，including 5 treatments with six replicates of each experiment.Compared with control group under normoxic and hypoxic，Exp.1 showed that VC significantly increased SOD to MDA ratio (P<0.05)，and down-regulated the mRNA transcription of HIF-1α/ VEGF/ VEGFR2 (P<0.05)；Exp.2 showed that DMOG significantly increased SOD to MDA ratio (P<0.05)，and down-regulated the mRNA transcription of HIF-1α and VEGFR2 (P<0.05) respectively，but significantly up-regulated the mRNA transcription of VEGF (P<0.05)，VC + DMOG significantly up-regulated the mRNA transcription of HIF-1α/VEGF/VEGFR2 (P<0.05)；Exp.3 showed that VC+H₂O₂+DMOG markedly up-regulated the mRNA transcription of HIF-1α/VEGF/VEGFR2 (P<0.01).These results suggested that VC could increase cellular antioxidantive ability，and regulate the relative expression of hypoxia genes based on the activity of proline hydroxylase and the redox state of broiler PASMCs.

CLC Number:

S852.2

CAO Lin，ZENG Qiu-feng，ZHANG Ke-ying，DING Xue-mei，BAI Shi-ping，LUO Yu-heng，WANG Jian-ping，XUAN Yue，SU Zhuo-wei. Molecular Mechanism of Vitamin C on Hypoxia Inducible Factor-1α mRNA Transcription of Broiler Pulmonary Artery Smooth Muscle Cells under Hypoxia Condition[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1033-1040.

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Molecular Mechanism of Vitamin C on Hypoxia Inducible Factor-1α mRNA Transcription of Broiler Pulmonary Artery Smooth Muscle Cells under Hypoxia Condition

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